bifidobacterium dentium gram stain


morphotypes. Gram-stained vaginal smear from a pregnant woman with intermediate vaginal flora (Nugent score of 4). 1 and 2). DNA-DNA hybridization with the BAN probe was only positive for samples from cattle and goats. No human fecal sample was positive for the BAN probe. The procedure is based on the detection of certain Bifidobacterium species in the samples. (28) designed species-specific 16S rDNA-targeted primers for the detection and quantification of predominant anaerobic bacteria in human and animal fecal samples. Some Bifidobacterium species are of human origin, whereas others are exclusively found in animals (3, 24). Yamamoto et al. Multiple alignments with all of the sequences were performed by using the GCG sequence analysis software package (University of Wisconsin, Madison). infections require further examination based on the study of more patients. The intermediate vaginal flora type here likely refers to a mixture of Lactobacillus and Gardnerella or Bacteroides spp. Bifidobacterial DNA was detected by qRTi-PCR in 22 out of 23 breast milk samples at a range between 40 and 10,000 16S rRNA gene copies per ml (Table 2). Figure 1. The optimum temperature for each probe was established as 60°C. DNA extraction from pure cultures.DNA extraction from bifidobacteria was performed by using a previously described protocol (11). The authors declare no conflicts of interest regarding the publication of this paper. Thank you for sharing this Applied and Environmental Microbiology article. The following amplification program was used: one cycle consisted of 95°C for 2 min; this was followed by 35 cycles of 94°C for 1 min, 67°C for 3 min, and 72°C for 4 min, with a final cycle of 72°C for 7 min. DNA-DNA hybridization with DIG-labeled probes.The 16S rRNA gene amplimers were vacuum blotted onto nylon membranes (Hybond-N; Amersham Pharmacia Biotech) by using the Minifold I (Schleicher & Schuell, Dassel, Germany). We are reporting two cases of mixed pyogenic infections due to Bifidobacterium sp. stream ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology. If preterm labor symptoms appear, the physician should consider prescribing penicillin in order to prevent a secondary-bacterial infection in the uterus. A protocol for the detection of the target species of the BAN and BDE probes in environmental samples was defined and evaluated. Of the 20 samples of adult feces analyzed, 5 showed positive hybridization for B. dentium with the BDE probe.

All of the samples of animal feces were positively amplified with the Bifidobacterium genus-specific primers, except for two samples of pig feces, one sample from a pig slaughterhouse, and one sample from a cattle slaughterhouse. PCR amplification.Each PCR mixture (50 μl) was composed of 50 mM KCl, 10 mM Tris-HCl (pH 8.3), 2 mM MgCl2, 0.1% Triton X-100, each deoxynucleoside triphosphate at a concentration of 200 μM, the Bifidobacterium genus-specific primers lm26 and lm3 (10) at a concentration of 0.3 μM, 2 μl of bacterial DNA extraction, and 2 U of Taq DNA polymerase (Eppendorf, Hamburg, Germany). The detection limit of the BAN probe was 104 CFU/ml. Sampling procedures.Twenty-nine samples of urban sewage were collected from the entrance to three wastewater treatment plants and from two hospital sewage collectors.
Especially Bifidobacterium bifidy and Bifidobacterium breve were present at higher concentrations and prevalence in the normal vagina than in the BV group, which may mean that they could be important for maintaining a healthy vaginal microenvironment [17].

Three experimental temperatures (Tm, Tm −5°C, and Tm, +5°C) were used to hybridize dot blot membranes with the target species and possible interfering species. Of the 29 samples of human sewage amplified by PCR, 27 gave a positive signal for DNA-DNA hybridization with the BDE probe, whereas none did so for hybridization with the BAN probe. Specificity of probes.Figure 1 shows the DNA-DNA hybridization results for 24 Bifidobacterium species when the probes BAN (Fig. Prehybridization and hybridization with the DIG-labeled probe, BAN, or BDE were performed as described by the manufacturer (Boehringer Mannheim) at 60°C. This vaginal smear type is a mixture of. Bacterial strains and growth conditions.Bacterial strains used in the present study are listed in Table 1. We do not have information on whether the pregnant women presenting with mid- or third trimester preterm PROM had been screened for BV in their first trimester for the purpose of preventing preterm birth.

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